When you organize your manuscript, the first thing
to consider is that the order of sections will be very different than the order
of items on you checklist.
An article begins
with the Title, Abstract and Keywords.
The article text
follows the IMRAD format, which
responds to the questions below:
- Introduction:
What did you/others do? Why did you do it?
- Methods:
How did you do it?
- Results:
What did you find?
- And
- Discussion:
What does it all mean?
The main text is
followed by the Conclusion, Acknowledgements, References and Supporting
Materials.
While this is the
published structure, however, we often use a different order when writing.
Steps to
organizing your manuscript
- Prepare
the figures and tables.
- Write
the Methods.
- Write up
the Results.
- Write
the Discussion.
Finalize the Results and Discussion before writing the introduction. This
is because, if the discussion is insufficient, how can you objectively
demonstrate the scientific significance of your work in the introduction?
- Write a
clear Conclusion.
- Write a
compelling introduction.
- Write
the Abstract.
- Compose a
concise and descriptive Title.
- Select Keywords for
indexing.
- Write
the Acknowledgements.
- Write up
the References.
Next, I'll review
each step in more detail. But before you set out to write a paper, there are
two important things you should do that will set the groundwork for the entire
process.
- The topic to be studied should be the first
issue to be solved. Define your hypothesis and objectives (These will go
in the Introduction.)
- Review the literature related to the topic and
select some papers (about 30) that can be cited in your paper (These will
be listed in the References.)
Finally, keep in
mind that each publisher has its own style guidelines and preferences, so
always consult the publisher's Guide for Authors.
Step 1: Prepare the figures and
tables
Remember "a
figure is worth a thousand words." Hence, illustrations, including figures
and tables, are the most efficient way to present your results. Your data are
the driving force of the paper, so your illustrations are critical!
How do you decide between
presenting your data as tables or figures? Generally, tables give the actual
experimental results, while figures are often used for comparisons of
experimental results with those of previous works, or with
calculated/theoretical values (Figure 1).
Figure 1. An example of the same data presented as
table or as figure. Depending in your objectives, you can show your data either
as table (if you wish to stress numbers) or as figure (if you wish to compare
gradients). Note: Never include vertical lines in a table.
Whatever your
choice is, no illustrations should duplicate the information described
elsewhere in the manuscript. Another important factor: figure and table legends
must be self-explanatory (Figure 2).
AB Figure 2
When presenting
your tables and figures, appearances count! To this end:
- Avoid crowded plots (Figure 3), using only
three or four data sets per figure; use well-selected scales.
- Think about appropriate axis label size
- Include clear symbols and data sets that are
easy to distinguish.
- Never include long boring tables (e.g.,
chemical compositions of emulsion systems or lists of species and
abundances). You can include them as supplementary material.
AB Figure 3
If you are using
photographs, each must have a scale marker, or scale bar, of professional
quality in one corner.
In photographs and
figures, use colour only when necessary when submitting to a print publication.
If different line styles can clarify the meaning, never use colours or other
thrilling effects or you will be charged with expensive fees. Of course, this
does not apply to online journals. For many journals, you can submit duplicate
figures: one in color for the online version of the journal and pdfs, and
another in black and white for the hardcopy journal (Figure 4).
AB Figure 4
Another common problem
is the misuse of lines and histograms. Lines joining data only can be used when
presenting time series or consecutive samples data (e.g., in a transect from
coast to offshore in Figure 5). However, when there is no connection between
samples or there is not a gradient, you must use histograms (Figure 5).
AB Figure 5
Sometimes,
fonts are too small for the journal. You must take this into account, or they
may be illegible to readers (Figure 6).
AB Figure 6
Finally, you must
pay attention to the use of decimals, lines, etc. (Figure 7)
Step 2: Write the Methods
This section
responds to the question of how the problem was studied. If your paper is
proposing a new method, you need to include detailed information so a
knowledgeable reader can reproduce the experiment.
However, do not
repeat the details of established methods; use References and Supporting
Materials to indicate the previously published procedures. Broad summaries or
key references are sufficient.
Reviewers will
criticize incomplete or incorrect methods descriptions and may recommend
rejection, because this section is critical in the process of reproducing your
investigation. In this way, all chemicals must be identified. Do not use
proprietary, unidentifiable compounds.
To this end, it's
important to use standard systems for numbers and nomenclature. For example:
- For chemicals, use the conventions of the International Union of Pure and Applied Chemistry and
the official recommendations of the IUPAC–IUB Combined Commission on
Biochemical Nomenclature.
- For species, use accepted taxonomical nomenclature (WoRMS: World Register of Marine
Species, ERMS:
European Register of Marine Species), and write them always in
italics.
- For units of measurement, follow the International System of Units (SI).
Present proper
control experiments and statistics used, again to make the experiment of
investigation repeatable.
List the methods
in the same order they will appear in the Results section, in the logical order
in which you did the research:
- Description of the site
- Description of the surveys or experiments
done, giving information on dates, etc.
- Description of the laboratory methods,
including separation or treatment of samples, analytical methods,
following the order of waters, sediments and biomonitors. If you have
worked with different biodiversity components start from the simplest
(i.e. microbes) to the more complex (i.e. mammals)
- Description of the statistical methods used
(including confidence levels, etc.)
In this section,
avoid adding comments, results, and discussion, which is a common error.
Length of the manuscript
Again, look at the journal's Guide
for Authors, but an ideal length for a manuscript is 25 to 40 pages, double
spaced, including essential data only. Here are some general guidelines:
- Title: Short
and informative
- Abstract: 1
paragraph (<250 words)
- Introduction: 1.5-2
pages
- Methods: 2-3
pages
- Results: 6-8
pages
- Discussion: 4-6
pages
- Conclusion: 1
paragraph
- Figures: 6-8
(one per page)
- Tables: 1-3
(one per page)
- References: 20-50
papers (2-4 pages)
Step 3: Write up the Results
This section
responds to the question "What have you found?" Hence, only
representative results from your research should be presented. The results
should be essential for discussion.
However, remember that
most journals offer the possibility of adding Supporting Materials, so use them
freely for data of secondary importance. In this way, do not attempt to
"hide" data in the hope of saving it for a later paper. You may lose
evidence to reinforce your conclusion. If data are too abundant, you can use
those supplementary materials.
Use sub-headings
to keep results of the same type together, which is easier to review and read.
Number these sub-sections for the convenience of internal cross-referencing,
but always taking into account the publisher's Guide for Authors.
For the data,
decide on a logical order that tells a clear story and makes it and easy to
understand. Generally, this will be in the same order as presented in the
methods section.
An important issue
is that you must not include references in this section; you are
presenting your results, so you cannot refer to others here.
If you refer to others, is because you are discussing your
results, and this must be included in the Discussion section.
Statistical rules
- Indicate the statistical tests used with all
relevant parameters: e.g., mean and standard deviation (SD): 44% (±3);
median and interpercentile range: 7 years (4.5 to 9.5 years).
- Use mean and standard deviation to report
normally distributed data.
- Use median and interpercentile range to report
skewed data.
- For numbers, use two significant digits unless
more precision is necessary (2.08, not 2.07856444).
- Never use percentages for very small samples
e.g., "one out of two" should not be replaced by 50%.
Step 4: Write the Discussion
Here you must
respond to what the results mean. Probably it is the easiest section to write,
but the hardest section to get right. This is because it is the most important
section of your article. Here you get the chance to sell your data. Take into
account that a huge numbers of manuscripts are rejected because the Discussion
is weak.
You need to make
the Discussion corresponding to the Results, but do not reiterate the results.
Here you need to compare the published results by your colleagues with yours
(using some of the references included in the Introduction). Never ignore work
in disagreement with yours, in turn, you must confront it and convince the
reader that you are correct or better.
Take into account
the following tips:
1. Avoid statements
that go beyond what the results can support.
2. Avoid unspecific expressions such as "higher
temperature", "at a lower rate", "highly significant".
Quantitative descriptions are always preferred (35ºC, 0.5%, p<0.001,
respectively).
3. Avoid sudden introduction of new
terms or ideas; you must present everything in the introduction, to be confronted
with your results here.
4. Speculations on possible
interpretations are allowed, but these should be rooted in fact, rather than
imagination. To achieve good interpretations think about:
- How
do these results relate to the original question or objectives outlined in
the Introduction section?
- Do the
data support your hypothesis?
- Are
your results consistent with what other investigators have reported?
- Discuss
weaknesses and discrepancies. If your results were unexpected, try to
explain why
- Is
there another way to interpret your results?
- What
further research would be necessary to answer the questions raised by your
results?
- Explain
what is new without exaggerating
5.
Revision of Results and Discussion is not just paper work. You
may do further experiments, derivations, or simulations. Sometimes you cannot
clarify your idea in words because some critical items have not been studied
substantially.
Step 5: Write a clear
Conclusion
This section shows
how the work advances the field from the present state of knowledge. In some
journals, it's a separate section; in others, it's the last paragraph of the
Discussion section. Whatever the case, without a clear conclusion section,
reviewers and readers will find it difficult to judge your work and whether it
merits publication in the journal.
A common error in
this section is repeating the abstract, or just listing experimental results.
Trivial statements of your results are unacceptable in this section.
You should provide
a clear scientific justification for your work in this section, and indicate
uses and extensions if appropriate. Moreover, you can suggest future
experiments and point out those that are underway.
You can propose
present global and specific conclusions, in relation to the objectives included
in the introduction
Step 6: Write a compelling
Introduction
This is your
opportunity to convince readers that you clearly know why your work is useful.
A good
introduction should answer the following questions:
- What is the problem to be solved?
- Are there any existing solutions?
- Which is the best?
- What is its main limitation?
- What do you hope to achieve?
Editors like to
see that you have provided a perspective consistent with the nature of the
journal. You need to introduce the main scientific publications on which your
work is based, citing a couple of original and important works, including
recent review articles.
However, editors
hate improper citations of too many references irrelevant to the work, or
inappropriate judgments on your own achievements. They will think you have no
sense of purpose.
Here are some
additional tips for the introduction:
- Never use more words than necessary (be
concise and to-the-point). Don't make this section into a history lesson.
Long introductions put readers off.
- We all know that you are keen to present your
new data. But do not forget that you need to give the whole picture at
first.
- The introduction must be organized from the
global to the particular point of view, guiding the readers to your
objectives when writing this paper.
- State the purpose of the paper and research
strategy adopted to answer the question, but do not mix introduction with
results, discussion and conclusion. Always keep them separate to ensure
that the manuscript flows logically from one section to the next.
- Hypothesis and objectives must be clearly
remarked at the end of the introduction.
- Expressions such as "novel,"
"first time," "first ever," and
"paradigm-changing" are not preferred. Use them sparingly.
Step 7: Write the Abstract
The abstract tells
prospective readers what you did and what the important findings in your
research were. Together with the title, it's the advertisement of your article.
Make it interesting and easily understood without reading the whole
article. Avoid using jargon, uncommon abbreviations and references.
You must be
accurate, using the words that convey the precise meaning of your research. The
abstract provides a short description of the perspective and purpose of your
paper. It gives key results but minimizes experimental details. It is very
important to remind that the abstract offers a short description of the
interpretation/conclusion in the last sentence.
A clear abstract
will strongly influence whether or not your work is further considered.
However, the
abstracts must be keep as brief as possible. Just check the 'Guide for authors'
of the journal, but normally they have less than 250 words. Here's a good example on
a short abstract.
In an abstract,
the two whats are essential. Here's an example from an article I
co-authored in Ecological Indicators:
- What has been done? "In recent years, several benthic biotic
indices have been proposed to be used as ecological indicators in
estuarine and coastal waters. One such indicator, the AMBI (AZTI Marine
Biotic Index), was designed to establish the ecological quality of
European coasts. The AMBI has been used also for the determination of the
ecological quality status within the
context of the European Water Framework Directive. In this contribution,
38 different applications including six new case studies (hypoxia
processes, sand extraction, oil platform impacts, engineering works,
dredging and fish aquaculture) are presented."
- What are the main findings? "The results show the response of the
benthic communities to different disturbance sources in a simple way.
Those communities act as ecological indicators of the 'health' of the
system, indicating clearly the gradient associated with the
disturbance."
Step 8: Compose a concise and descriptive title
The title must explain what the paper is broadly
about. It is your first (and probably only) opportunity to attract the reader's
attention. In this way, remember that the first readers are the Editor and the
referees. Also, readers are the potential authors who will cite your article,
so the first impression is powerful!
We are all flooded by publications, and readers
don't have time to read all scientific production. They must be selective, and
this selection often comes from the title.
Reviewers will check whether the title is specific
and whether it reflects the content of the manuscript. Editors hate titles that
make no sense or fail to represent the subject matter adequately. Hence, keep
the title informative and concise (clear, descriptive, and not too long). You
must avoid technical jargon and abbreviations, if possible. This is because you
need to attract a readership as large as possible. Dedicate some time to think
about the title and discuss it with your co-authors.
Here you can see some examples of original titles,
and how they were changed after reviews and comments to them:
Example 1
- Original title: Preliminary
observations on the effect of salinity on benthic community distribution
within a estuarine system, in the North Sea
- Revised title: Effect
of salinity on benthic distribution within the Scheldt estuary (North Sea)
- Comments: Long
title distracts readers. Remove all redundancies such as "studies
on," "the nature of," etc. Never use expressions such as
"preliminary." Be precise.
Example 2
- Original title: Action
of antibiotics on bacteria
- Revised title: Inhibition
of growth of Mycobacterium tuberculosis by streptomycin
- Comments: Titles
should be specific. Think about "how will I search for this piece of
information" when you design the title.
Example 3
- Original title: Fabrication
of carbon/CdS coaxial nanofibers displaying optical and electrical
properties via electrospinning carbon
- Revised title: Electrospinning
of carbon/CdS coaxial nanofibers with optical and electrical properties
- Comments: "English
needs help. The title is nonsense. All materials have properties of all
varieties. You could examine my hair for its electrical and
optical properties! You MUST be specific. I haven't read the paper but I
suspect there is something special about these properties, otherwise why
would you be reporting them?" – the Editor-in-Chief.
Try to avoid this kind of response!
Step 9: Select keywords for indexing
Keywords are used for indexing your paper. They are
the label of your manuscript. It is true that now they are less used by
journals because you can search the whole text. However, when looking for
keywords, avoid words with a broad meaning and words already included in the
title.
Some journals require that the keywords are not
those from the journal name, because it is implicit that the topic is that. For
example, the journal Soil Biology & Biochemistry requires
that the word "soil" not be selected as a keyword.
Only abbreviations firmly established in the field
are eligible (e.g., TOC, CTD), avoiding those which are not broadly used (e.g.,
EBA, MMI).
Again, check the Guide for Authors and look at the
number of keywords admitted, label, definitions, thesaurus, range, and other
special requests.
Step 10: Write the Acknowledgements
Here, you can thank people who have contributed to
the manuscript but not to the extent where that would justify authorship. For
example, here you can include technical help and assistance with writing and
proofreading. Probably, the most important thing is to thank your funding
agency or the agency giving you a grant or fellowship.
In the case of European projects, do not forget to
include the grant number or reference. Also, some institutes include the number
of publications of the organization, e.g., "This is publication number 657
from AZTI-Tecnalia."
Step 11: Write up the References
Typically, there are more mistakes in the
references than in any other part of the manuscript. It is one of the most
annoying problems, and causes great headaches among editors. Now, it is easier
since to avoid these problem, because there are many available tools.
In the text, you must cite all the scientific
publications on which your work is based. But do not over-inflate the
manuscript with too many references – it doesn't make a better manuscript!
Avoid excessive self-citations and excessive citations of publications from the
same region.
Minimize personal
communications, do not include unpublished observations, manuscripts submitted
but not yet accepted for publication, publications that are not peer reviewed,
grey literature, or articles not published in English.
As I have mentioned, you will find the most
authoritative information for each journal’s policy on citations when you
consult the journal's Guide for Authors. In general, you should minimize
personal communications, and be mindful as to how you include unpublished
observations. These will be necessary for some disciplines, but consider
whether they strengthen or weaken your paper. You might also consider articles published on research networks prior
to publication, but consider balancing these citations with citations of
peer-reviewed research. When citing research in languages other than English,
be aware of the possibility that not everyone in the review process will speak
the language of the cited paper and that it may be helpful to find a
translation where possible.
You can use any software, such as EndNote or Mendeley,
to format and include your references in the paper. Most journals have now the
possibility to download small files with the format of the references, allowing
you to change it automatically. Also, Elsevier's Your Paper Your Way program waves
strict formatting requirements for the initial submission of a manuscript as
long as it contains all the essential elements being presented here.
Make the reference list and the in-text citation
conform strictly to the style given in the Guide for Authors. Remember that
presentation of the references in the correct format is the responsibility of
the author, not the editor. Checking the format is normally a large job for the
editors. Make their work easier and they will appreciate the effort.
Finally, check the following:
- Spelling
of author names
- Year
of publications
- Usages
of "et al."
- Punctuation
- Whether
all references are included
By Thesis Doctor
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